By combining the finest technology and passion,
INCYTOИ® creates added value for the customer.
AUTOMATED INVERTED MICROSCOPY SYSTEM
We utilize especially for INCYTOИ® developed inverted microscope technology from TILL I.D. GmbH (Munich, Germany). The microscopes are smartly reduced to the essential functions and therefore optimizes in size, weight and functional range. Care was taken to use as few moving parts as possible, resulting in greater mechanical durability. The only moving part is the ultra-fast voice-coil actuator for the objective lens Z axis (focus), which has a sub-micrometer resolution.
In combination with the 4k camera, high resolution inverted microscopy on living cells can be performed. Parallel adapted read-out technology for optochemical sensors (fluorescence lifetime measuring) can be guided through the microscope optics without interfering the imaging. This makes it a powerful tool for imaging and sensor read-out in parallel.
The microscope system is completed by INCYTOИ® self-developed components. On the one hand, a microscope illuminating arrangement, which produces controllable light patterns, and, on the other hand, a micrometer accurate YX-stage, which positions the microscope exactly under the software-defined location.
OPTOCHEMICAL pH AND O₂ MEASURING
INCYTOИ® integrates non-invasive label-free real-time sensor technology for media pH and dissolved O₂ in its platform solutions. This accurate measuring method is provided by PreSens Precision Sensing GmbH (Regensburg, Germany) and adapted to our microscope system. The non-invasive sensors change their fluorescence lifetime in dependence of the analyte and can be read-out by therefore developed measuring technology in real-time. In combination with the INCYTOИ® fluidic technology, this makes it possible to monitor the cellular metabolic key parameters glycolysis and oxidative phosphorylation in living cells without any influence on these.
Impedance sensing is a non-invasive technology to monitor cellular morphologic conditions. INCYTOИ® applies this powerful technology in its measuring systems to monitor cellular proliferation and response to treatment in real-time. Therefore, the cells are cultivated on microelectrodes which direct a harmless electrical microcurrent through the cells and the surrounding medium. As result, an electrical alternating current resistance (impedance) can be monitored by precise measuring technology (self-developed by INCYTOИ®). This impedance changes depending on the morphologic status of the cell.
MULTICHANNEL PIPETTE ROBOTIC
INCYTOИ® developed a special so called “Selective Compliance Assembly Robot Arm” (SCARA) with multichannel pipetting head for automated media manipulation. This very compact unit can fully automated and precisely add, remove and mix up to 200µl liquid media to and from culture well plates by utilizing sterile micropipette tips. This puts it in the position to support cell-based assays from simple pipetting schemes like culture media exchange to complex tasks like accurate to the second multi-substance applications or washout experiments.
Changes that are triggered by something as small as cells can only be sensibly measured in a microenvironment. INCYTOИ® has integrated this technology into a multiwell plate, in which the cells can be examined and treated with low shear forces. In combination with our real-time sensors, reaction kinetics of different cell populations can be recorded in parallel.
CUSTOMIZED ATMOSPHERE CHAMBER TECHNOLOGY
The Atmosphere Chamber brings together all INCYTOИ® technology under its roof. This customized for INCYTOИ® manufactured key component can provide the fully automated atmospheric control (temperature, humidity, gas composition), which is essential for the accurate measuring of sensor equipment. Additionally, it generates a shelter against external influences for your biological samples and media.
RAPID 3D PROTOTYPING
Today, fast adapting development increments are demanded by the market. Therefore, INCYTOИ® implemented rapid 3D prototyping in its processes. Using high-precision resin SLA printing technology from Formlabs (Somerville, USA) we are able to manufacture functional prototypes with relatively low costs over night. This enables us to respond to necessary changes without any delays.